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JoVE Journal Developmental Biology
Maturação de cardiomiócitos estaminais humanas derivadas de células em Biowires Usando Electrical Stimulation
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Maturação de cardiomiócitos estaminais humanas derivadas de células em Biowires Usando Electrical Stimulation

Article DOI: 10.3791/55373-v • 10:11 min • May 6th, 2017
May 6th, 2017 •
1, 1,2,3,4
1Toronto General Research Institute, University Health Network, 2Institute of Biomaterials and Biomedical Engineering, University of Toronto, 3Heart & Stroke/Richard Lewar Centre of Excellence, University of Toronto, 4Laboratory of Medicine and Pathobiology, University of Toronto

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A plataforma biowire cardíaca é um método in vitro utilizado para amadurecer embrionário humano e cardiomiócitos derivadas de células estaminais pluripotentes induzidas (HPSC-CM) através da combinação de cultura de células tridimensional com estimulação eléctrica. Este manuscrito apresenta a configuração detalhada da plataforma biowire cardíaca.

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Developmental Biology 123 Edição Biowire células de cardiomiócitos engenharia de tecidos maturação pistas biomiméticos haste cultura de células 3D
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