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Journal
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Immunology and Infection
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简化的单细胞 TCR 分离和逆转录病毒载体的生成, 用于
体外
和
体内
表达人类 TCRs
JoVE Journal
Immunology and Infection
Author Produced
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JoVE Journal
Immunology and Infection
Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for
In Vitro
and
In Vivo
Expression of Human TCRs
Please note that all translations are automatically generated.
Click here for the English version.
简化的单细胞 TCR 分离和逆转录病毒载体的生成, 用于
体外
和
体内
表达人类 TCRs
DOI:
10.3791/55379-v
•
11:21 min
•
September 10, 2017
•
Maran L. Sprouse
,
Gabriele Blahnik
,
Thomas Lee
,
Natalie Tully
,
Pinaki Benarjee
,
Eddie A. James
,
Maria J. Redondo
,
Matthew L. Bettini
,
Maria Bettini
1
Department of Pediatrics, Section of Diabetes and Endocrinology, McNair Medical Institute
,
Baylor College of Medicine, Texas Children’s Hospital
,
2
Benaroya Research Institute at Virginia Mason
,
3
Center for Human Immunobiology
,
Baylor College of Medicine, Texas Children’s Hospital
,
4
Department of Pediatrics, Section of Diabetes and Endocrinology
,
Baylor College of Medicine, Texas Children’s Hospital
Chapters
00:00
Title
01:42
Single cell sort and reverse transcription reaction
03:37
Multiplex-nested PCR
08:56
Subcloning TCR alpha and beta chains
09:56
Verification of TCR expression
10:46
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
当前的协议结合了单细胞配对人类 TCR α和β链测序与与
体外
和
体内
TCR 表达相兼容的反转录病毒载体的生成。
Tags
Single Cell TCR Isolation
TCR Alpha And Beta Repertoire
TCR Cloning
Multiplex PCR
Nested PCR
Chimeric TCR Construct
Single-cell PCR
Reverse Transcription
T Cell Sorting
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