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Journal
/
Immunology and Infection
/
合理化単細胞 TCR 分離し
体外
の Retroviral ベクトルの生成、人間 Tcr の
生体内で
発現
JoVE Journal
Immunology and Infection
Author Produced
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JoVE Journal
Immunology and Infection
Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for
In Vitro
and
In Vivo
Expression of Human TCRs
Please note that all translations are automatically generated.
Click here for the English version.
合理化単細胞 TCR 分離し
体外
の Retroviral ベクトルの生成、人間 Tcr の
生体内で
発現
DOI:
10.3791/55379-v
•
11:21 min
•
September 10, 2017
•
Maran L. Sprouse
,
Gabriele Blahnik
,
Thomas Lee
,
Natalie Tully
,
Pinaki Benarjee
,
Eddie A. James
,
Maria J. Redondo
,
Matthew L. Bettini
,
Maria Bettini
1
Department of Pediatrics, Section of Diabetes and Endocrinology, McNair Medical Institute
,
Baylor College of Medicine, Texas Children’s Hospital
,
2
Benaroya Research Institute at Virginia Mason
,
3
Center for Human Immunobiology
,
Baylor College of Medicine, Texas Children’s Hospital
,
4
Department of Pediatrics, Section of Diabetes and Endocrinology
,
Baylor College of Medicine, Texas Children’s Hospital
Chapters
00:00
Title
01:42
Single cell sort and reverse transcription reaction
03:37
Multiplex-nested PCR
08:56
Subcloning TCR alpha and beta chains
09:56
Verification of TCR expression
10:46
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
単一のセル対人間 TCR アルファとベータ鎖シーケンスで現在のプロトコル結合合理化レトロウイルスベクター
の in vitro
と
in vivo
の TCR 式と互換性の世代です。
Tags
Single Cell TCR Isolation
TCR Alpha And Beta Repertoire
TCR Cloning
Multiplex PCR
Nested PCR
Chimeric TCR Construct
Single-cell PCR
Reverse Transcription
T Cell Sorting
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