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Journal / Immunology and Infection / Gestroomlijnd eencellige TCR isolatie en…
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Immunology and Infection
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JoVE Journal Immunology and Infection
Gestroomlijnd eencellige TCR isolatie en generatie van retrovirale vectoren voor In Vitro en In Vivo expressie van menselijke TCRs
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Gestroomlijnd eencellige TCR isolatie en generatie van retrovirale vectoren voor In Vitro en In Vivo expressie van menselijke TCRs

Article DOI: 10.3791/55379-v • 11:21 min • September 10th, 2017
September 10th, 2017 •
1, 2, 1, 1, 3, 2, 4, 1, 1
1Department of Pediatrics, Section of Diabetes and Endocrinology, McNair Medical Institute, Baylor College of Medicine, Texas Children's Hospital, 2Benaroya Research Institute at Virginia Mason, 3Center for Human Immunobiology, Baylor College of Medicine, Texas Children's Hospital, 4Department of Pediatrics, Section of Diabetes and Endocrinology, Baylor College of Medicine, Texas Children's Hospital

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De huidige protocol combineert eencellige gekoppeld menselijke TCR alpha en beta keten sequencing met gestroomlijnd generatie van retrovirale vectoren compatibel met in vitro en in vivo TCR expressie.

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Immunologie kwestie 127 T-cel T cel receptor TCR PCR sequencing retrovirale één cel menselijke in vivoen in vitro TCR expressie
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