A Protocol to Characterize the Morphological Changes of Clostridium difficile in Response to Antibiotic Treatment

Bradley Endres; Eug&#233;nie Bass&#232;res; Tasnuva Rashid; Long Chang; M. Jahangir Alam; Kevin W Garey

doi:10.3791/55383

Journal

/

Immunology and Infection

/

形態変化を特徴付けるプロトコルクロストリジウム・ディフィシル抗生物質治療に対する反応

A Protocol to Characterize the Morphological Changes of <em>Clostridium difficile</em> in Response to Antibiotic Treatment

JoVE Journal
Immunology and Infection

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Immunology and Infection

A Protocol to Characterize the Morphological Changes of Clostridium difficile in Response to Antibiotic Treatment

形態変化を特徴付けるプロトコルクロストリジウム・ディフィシル抗生物質治療に対する反応

Please note that all translations are automatically generated. Click here for the English version.

8,923 Views

•

12:58 min

•

May 25, 2017

DOI:

10.3791/55383-v

DOI:

10.3791/55383-v

•

12:58 min

May 25, 2017

•

5 Views

Bradley Endres¹, Eugénie Bassères¹, Tasnuva Rashid¹, Long Chang², M. Jahangir Alam¹, Kevin W Garey¹

¹Department of Pharmacy Practice and Translational Research,University of Houston College of Pharmacy, ²Department of Electrical and Computer Engineering,University of Houston

Transcript

Summary

Antibiotic efficacy is most commonly determined by conducting killing kinetic studies and measuring colony forming units (CFUs). By integrating scanning electron microscopy (SEM) with these standard methods, we can distinguish the pharmacological effects of treatment between different antibiotics.