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JoVE Journal Neuroscience
Utarbeidelse av ikke-menneskelige primater hjernevev for Pre-embedding Immunohistokjemi og elektronmikroskopi
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Utarbeidelse av ikke-menneskelige primater hjernevev for Pre-embedding Immunohistokjemi og elektronmikroskopi

Article DOI: 10.3791/55397-v • 11:55 min • April 3rd, 2017
April 3rd, 2017 •
1,2, 1
1Centre de recherche de l'Institut universitaire en santé mentale de Québec, Department of Psychiatry and Neuroscience, Université Laval, 2Centre de recherche du CHU Sainte-Justine

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Her gir vi en enkel, billig, og tidsbesparende protokoll for kjemisk å feste primat hjernevev med acrolein fiksativ, slik at for langtidskonservering som er kompatibel med forhånds innleiring immunhistokjemi for transmisjonselektronmikroskopi.

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Neuroscience utgave 122 transmisjonselektronmikroskopi ape neuroanatomy immunohistokjemi antistoffer akrolein transkardiakperfusjon forankring ultramikrotom
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