Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Bioengineering / The Use of a β-lactamase-based…
JoVE Journal
Bioengineering

A subscription to JoVE is required to view this content.

JoVE Journal Bioengineering
The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
Assessing Collagen and Elastin Pressure-dependent Microarchitectures in Live, Human Resistance Arteries by Label-free Fluorescence Microscopy
Journal (Bioengineering)
Assessing Collagen and Elastin Pressure-dependent Microarchitectures in Live, Human Resistance Arteries by Label-free Fluorescence Microscopy
A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
Journal (Bioengineering)
A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
 

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

Article DOI: 10.3791/55414-v • 08:06 min • February 1st, 2018
February 1st, 2018 •
*1, *1, 2, 1, 1, 1, 1, 3, 1, 4
1Life Science Department, University of Liège, 2Institute of Condensed Matter and Nanoscience, Catholic University of Louvain, 3Laboratory of Clinical Microbiology, Catholic University of Louvain, 4Biotechnology Department, CER Group
* These authors contributed equally

Chapters

Summary
Automatic Translation

In this work, we report a new method to study protein-protein interactions using a conductimetric biosensor based on the hybrid β-lactamase technology. This method relies on release of protons upon hydrolysis of β-lactams.

Tags

Conductimetric Biosensor Assay Biomolecular Interactions Beta-lactamase Technology Antibody Antigen Interactions Chimeric Beta-lactamase Protein Proton Release Immunosensors Sensory Assays Protein Sample Preparation Solution Preparation Electrode Preparation Solution Electrode Regeneration Solution
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter