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JoVE Journal Bioengineering
Brug af en β-lactamase-baseret Conductimetric Biosensor Assay til påvisning Biomolekylær interaktioner
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Brug af en β-lactamase-baseret Conductimetric Biosensor Assay til påvisning Biomolekylær interaktioner

Article DOI: 10.3791/55414-v • 08:06 min • February 1st, 2018
February 1st, 2018 •
*1, *1, 2, 1, 1, 1, 1, 3, 1, 4
1Life Science Department, University of Liège, 2Institute of Condensed Matter and Nanoscience, Catholic University of Louvain, 3Laboratory of Clinical Microbiology, Catholic University of Louvain, 4Biotechnology Department, CER Group
* These authors contributed equally

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I dette arbejde rapportere vi en ny metode til at undersøge protein-protein interaktioner ved hjælp af en conductimetric biosensor baseret på β-lactamase Hybridteknologien. Denne metode bygger på frigivelse af protoner ved hydrolyse af β-lactamer.

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Bioteknologi sag 132 β-lactamase hybrid protein teknologi (BHP) conductimetric biosensor nanobodies molekylære interaktioner Lysozym
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