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Neuroscience

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使用荧光素二乙酸酯 - 碘化丙啶双重染色在小脑颗粒神经元培养中评估神经元活力
 
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使用荧光素二乙酸酯 - 碘化丙啶双重染色在小脑颗粒神经元培养中评估神经元活力

Article DOI: 10.3791/55442-v 08:53 min May 10th, 2017
May 10th, 2017

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Summary

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Click here for the English version.

该协议描述了如何使用荧光素二乙酸酯(FDA)和碘化丙啶(PI)双重染色在培养的小脑颗粒神经元中准确测量神经元活力,这是一种初级神经元培养物,用作神经科学和神经药理学研究中的体外模型。

Tags

神经科学,第123期,小脑颗粒神经元,荧光素二乙酸酯,碘化丙啶,细胞活力,胶质细胞,荧光显微镜
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