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Profilage Redox cellulaire utilisant une microscopie haute teneur
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Profilage Redox cellulaire utilisant une microscopie haute teneur

Article DOI: 10.3791/55449-v • 11:37 min • May 14th, 2017
May 14th, 2017 •
1,2, 1,2, 1, 3, 1,2
1Laboratory of Cell Biology and Histology, Department of Veterinary Sciences, University of Antwerp, 2Cell Systems and Imaging Research Group (CSI), Department of Molecular Biotechnology, Ghent University, 3Department of Biochemistry , Radboud Institute for Molecular Life Sciences, Radboud University Medical Center

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Cet article présente un flux de travail de microscopie à contenu élevé pour la quantification simultanée des niveaux de ROS intracellulaires, ainsi que le potentiel de la membrane mitochondriale et la morphologie - communément appelée morphofonction mitochondriale - dans les cellules adhérentes vivantes en utilisant les molécules rapporteurs fluorescentes perméables aux cellules 5- (et- 6) -chlorométhyl-2 ', 7'-dichlorodihydrofluorescéine diacétate, ester acétylique (CM-H2RFDA) et tétraméthylrhodamine méthylester (TMRM).

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Biologie cellulaire numéro 123 Espèces d'oxygène réactives Mitochondrie Morphofunction mitochondriale Imagerie cellulaire en direct Microscopie haute teneur Microscopie fluorescente Microscopie quantitative multiparamétrique Biologie Redox
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