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Cellulär redoxprofilering med hög innehållsmikroskopi
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Cellulär redoxprofilering med hög innehållsmikroskopi

Article DOI: 10.3791/55449-v • 11:37 min • May 14th, 2017
May 14th, 2017 •
1,2, 1,2, 1, 3, 1,2
1Laboratory of Cell Biology and Histology, Department of Veterinary Sciences, University of Antwerp, 2Cell Systems and Imaging Research Group (CSI), Department of Molecular Biotechnology, Ghent University, 3Department of Biochemistry , Radboud Institute for Molecular Life Sciences, Radboud University Medical Center

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Detta papper presenterar ett högt innehållsmikroskopi-arbetsflöde för samtidig kvantifiering av intracellulära ROS-nivåer, såväl som mitokondriell membranpotential och morfologi - gemensamt betecknad som mitokondriell morfofunktion - i levande vidhäftande celler med användning av cell-permeant fluorescerande reportermolekyler 5- (och- 6) -klorometyl-2 ', 7'-diklorodihydrofluoresceindiacetat, acetylester (CM-H2 DCFDA) och tetrametylrhodamin-metylester (TMRM).

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Cellbiologi Utgåva 123 Reaktiva Oxygenarter Mitokondrier Mitokondriell Morfofunktion Live Cell Imaging Höghaltsmikroskopi Fluorescensmikroskopi Kvantitativ Multiparametrisk Mikroskopi Redoxbiologi
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