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Journal / Genetics / Ribosomal DNA에 따라 RNA 샘플에서 DNA 오염…
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Genetics

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JoVE Journal Genetics
Ribosomal DNA에 따라 RNA 샘플에서 DNA 오염 평가
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Ribosomal DNA에 따라 RNA 샘플에서 DNA 오염 평가

Article DOI: 10.3791/55451-v • 13:16 min • January 22nd, 2018
January 22nd, 2018 •
1, 1, 2, 3, 4
1Genetic and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources University (SANRU), 2Department of Industrial and Environmental Biotechnology, National Institute of Genetics Engineering and Biotechnology (NIGEB), 3Department of Plant Breeding and Biotechnology, Gorgan University of Agricultural Sciences & Natural Resources, 4RG Abiotic Stress Genomics/ RG Heterosis, Department Molecular Genetics, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)

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여기, 선물이 RNA 샘플에 게놈 DNA (gDNA) 오염 추적에 대 한 프로토콜. 제시 방법 뇌관 특정 ribosomal DNA (rDNA) 유전자의 내부 베낀된 공백 지역 (ITS)을 활용합니다. 메서드는 대부분의 진핵생물과 원핵생물에서 DNA 오염의 안정적이 고 민감한 검출에 적합 합니다.

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유전학 문제 131 정량 실시간 정량 Pcr 유전자 표정 분석 DNA 오염 분석 결과 ribosomal DNA (rDNA) ribosomal RNA (rRNA) Poaceae 원핵생물 진핵생물 보편적인 뇌관
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