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Journal / Genetics / Valutazione della contaminazione da DNA a RNA…
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Genetics

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JoVE Journal Genetics
Valutazione della contaminazione da DNA a RNA campioni basati sul DNA Ribosomal
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Valutazione della contaminazione da DNA a RNA campioni basati sul DNA Ribosomal

Article DOI: 10.3791/55451-v • 13:16 min • January 22nd, 2018
January 22nd, 2018 •
1, 1, 2, 3, 4
1Genetic and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources University (SANRU), 2Department of Industrial and Environmental Biotechnology, National Institute of Genetics Engineering and Biotechnology (NIGEB), 3Department of Plant Breeding and Biotechnology, Gorgan University of Agricultural Sciences & Natural Resources, 4RG Abiotic Stress Genomics/ RG Heterosis, Department Molecular Genetics, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)

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Qui, presentiamo un protocollo per l'analisi genomica contaminazione da DNA (gDNA) in campioni di RNA. Il metodo proposto utilizza gli iniettori specifici per la regione di distanziatore interno trascritti (ITS) di geni ribosomali DNA (rDNA). Il metodo è adatto per il rilevamento affidabile e sensibile di contaminazione da DNA nella maggior parte dei eucarioti e procarioti.

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Genetica problema 131 qPCR RT-qPCR analisi dell'espressione genica analisi di contaminazione del DNA DNA ribosomiale (rDNA) RNA ribosomiale (rRNA) Poaceae prokaryote eucariote primer universale
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