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Journal / Genetics / Vurdering af DNA forurening i RNA prøver baseret…
JoVE Journal
Genetics

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JoVE Journal Genetics
Vurdering af DNA forurening i RNA prøver baseret på ribosomale DNA
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Vurdering af DNA forurening i RNA prøver baseret på ribosomale DNA

Article DOI: 10.3791/55451-v • 13:16 min • January 22nd, 2018
January 22nd, 2018 •
1, 1, 2, 3, 4
1Genetic and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources University (SANRU), 2Department of Industrial and Environmental Biotechnology, National Institute of Genetics Engineering and Biotechnology (NIGEB), 3Department of Plant Breeding and Biotechnology, Gorgan University of Agricultural Sciences & Natural Resources, 4RG Abiotic Stress Genomics/ RG Heterosis, Department Molecular Genetics, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)

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Vi præsenterer her, en protokol for sporing genomisk DNA (gDNA) forurening i RNA prøver. Metoden præsenteres udnytter primere specifikke for regionen indre transskriberede spacer (ITS) i ribosomale DNA (rDNA) gener. Metoden er velegnet til pålidelige og følsomme påvisning af DNA forurening i de fleste eukaryoter og prokaryoter.

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Genetik sag 131 qPCR RT-qPCR gene expression analyse DNA forurening assay ribosomale DNA (rDNA) ribosomale RNA (rRNA) græs-familien prokaryot eukaryote universal primer
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