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Journal / Genetics / Bedömning av DNA kontaminering i RNA-prover…
JoVE Journal
Genetics

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JoVE Journal Genetics
Bedömning av DNA kontaminering i RNA-prover baserat på Ribosomal DNA
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Bedömning av DNA kontaminering i RNA-prover baserat på Ribosomal DNA

Article DOI: 10.3791/55451-v • 13:16 min • January 22nd, 2018
January 22nd, 2018 •
1, 1, 2, 3, 4
1Genetic and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources University (SANRU), 2Department of Industrial and Environmental Biotechnology, National Institute of Genetics Engineering and Biotechnology (NIGEB), 3Department of Plant Breeding and Biotechnology, Gorgan University of Agricultural Sciences & Natural Resources, 4RG Abiotic Stress Genomics/ RG Heterosis, Department Molecular Genetics, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)

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Här presenterar vi ett protokoll för att spåra genomisk DNA (gDNA) kontaminering i RNA-prover. Den presenterade metoden använder tredjeparts primers som är specifika för regionen inre transkriberas spacer (ITS) ribosomal DNA (rDNA) gener. Metoden passar för tillförlitlig och känslig detektion av DNA kontaminering i de flesta Eukaryoter och prokaryoter.

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Genetik fråga 131 qPCR RT-qPCR gen uttryck analys DNA kontaminering assay ribosomal DNA (rDNA) ribosomal RNA (rRNA) Poaceae prokaryoter Eukaryoter universal primer
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