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Journal / Biochemistry / Tidsupplöst elektrosprayjonisering Väte-deuterium …
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JoVE Journal Biochemistry
Tidsupplöst elektrosprayjonisering Väte-deuterium Exchange-masspektrometri för att studera Protein Structure and Dynamics
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Tidsupplöst elektrosprayjonisering Väte-deuterium Exchange-masspektrometri för att studera Protein Structure and Dynamics

Article DOI: 10.3791/55464-v • 09:18 min • April 17th, 2017
April 17th, 2017 •
1, 1, 1, 1, 1, 1,2,3
1Department of Chemistry, York University, 2The Centre for Research in Mass Spectrometry, York University, 3The Centre for Research on Biomolecular Interactions, York University

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Konforma flexibilitet spelar en avgörande roll i proteinfunktion. Häri beskriver vi användning av tidsupplöst elektrosprayjoniseringsmasspektrometri kopplad till väte-deuterium utbyte mot sondering de snabba strukturella förändringar som driver funktionen i ordnade och oordnade proteiner.

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Biokemi tidsupplöst elektrosprayjonisering masspektrometri väte-deuterium utbyte proteindynamik inneboende oordnade proteiner mikrofluidik
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