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Immunology and Infection

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Præcision-cut muselunge Skiver at visualisere Levende Pulmonal dendritiske celler
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Præcision-cut muselunge Skiver at visualisere Levende Pulmonal dendritiske celler

Article DOI: 10.3791/55465-v • 09:33 min • April 5th, 2017
April 5th, 2017 •
1, 1, 1, 1
1Immunity, Inflammation, and Disease Laboratory, Division of Intramural Research, National Institute of Environmental Health Sciences, NIH

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Vi beskriver en fremgangsmåde til frembringelse Precision-cut Lung Skiver (PCLS) og immunfarvning dem at visualisere lokaliseringen af ​​forskellige immun celletyper i lungen. Vores protokol kan udvides til at visualisere placeringen og funktionen af ​​mange forskellige celletyper under en række betingelser.

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Immunologi levende celler præcision cut lungesnit lunge dendritiske celler lokalisering konfokal mikroskopi
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