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Immunology and Infection

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JoVE Journal Immunology and Infection
Precision-cut Maus Lung Slices Live-Pulmonary Dendritische Zellen sichtbar zu machen
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Precision-cut Maus Lung Slices Live-Pulmonary Dendritische Zellen sichtbar zu machen

Article DOI: 10.3791/55465-v • 09:33 min • April 5th, 2017
April 5th, 2017 •
1, 1, 1, 1
1Immunity, Inflammation, and Disease Laboratory, Division of Intramural Research, National Institute of Environmental Health Sciences, NIH

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Wir beschreiben ein Verfahren zur Erzeugung von Precision-cut Lung Slices (PCLS) und immunostaining sie die Lokalisierung verschiedenen Immunzelltypen in der Lunge sichtbar zu machen. Unser Protokoll kann erweitert werden, um die Position und Funktion vieler verschiedenen Zelltypen unter einer Vielzahl von Bedingungen zu visualisieren.

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Immunology Ausgabe 122 lebenden Zellen Präzisionsschnitt Lungenschnitten Lungen- dendritische Zellen Lokalisierung konfokale Mikroskopie
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