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Ved hjælp pHluorin-mærkede receptorer til Monitor subcellulære lokalisering og handel
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Ved hjælp pHluorin-mærkede receptorer til Monitor subcellulære lokalisering og handel

Article DOI: 10.3791/55466-v • 09:59 min • March 16th, 2017
March 16th, 2017 •
1, 2, 1
1Department of Chemistry, University of Kentucky, 2Department of Biomedical Sciences, Marshall University

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Mærkning det ekstracellulære domæne af et membranprotein med en pH-følsom fluorophor, superecliptic pHluorin (SEP), giver mulighed for subcellulære lokalisering, udtryk, og menneskehandel, der skal fastlægges. Imaging SEP mærkede proteiner med total indre refleksion fluorescensmikroskopi (TIRFM) gør kvantificeringen af ​​proteinniveauer i det perifere ER og plasmamembranen.

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Biofysik Superecliptic pHluorin receptor membran menneskehandel subcellulære lokalisering plasmamembranen indsættelse total intern refleksion fluorescensmikroskopi membranprotein opregulering
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