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Utilizando Receptores pHluorin-tag para monitorar subcelular Localização e Tráfico
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Utilizando Receptores pHluorin-tag para monitorar subcelular Localização e Tráfico

Article DOI: 10.3791/55466 • 09:59 min
March 16th, 2017 •
1, 2, 1
1Department of Chemistry, University of Kentucky, 2Department of Biomedical Sciences, Marshall University

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March 16th, 2017

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Rotular o domínio extracelular de uma proteína de membrana com um fluoróforo sensível ao pH, superecliptic pHluorin (SEP), permite a localização subcelular, expressão, e o tráfico de ser determinado. proteínas imagiologia SEP-rotulado com microscopia de fluorescência de reflexão total interna (TIRFM) permite a quantificação dos níveis da proteína na membrana do RE e periférico plasma.

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