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Journal / Biology / pHluorin etiketli Reseptör kullanarak Subselüler…
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pHluorin etiketli Reseptör kullanarak Subselüler Yerelleşme ve Ticareti Monitör
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pHluorin etiketli Reseptör kullanarak Subselüler Yerelleşme ve Ticareti Monitör

Article DOI: 10.3791/55466 • 09:59 min
March 16th, 2017 •
1, 2, 1
1Department of Chemistry, University of Kentucky, 2Department of Biomedical Sciences, Marshall University

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March 16th, 2017

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pH'ya duyarlı bir florofor ile bir membran proteinin hücre dışı alanını Etiketleme, superecliptic pHluorin (SEP), hücre içi lokalizasyonu, sentezleme ve trafik saptanmasına olanak sağlamaktadır. Görüntüleme toplam iç yansıma floresan mikroskobu (TIRFM) proteinleri Eylül-etiketli periferal ER ve plazma membranı protein seviyelerinin ölçümü sağlar.

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