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Utnytte pHluorin-merket reseptorer å overvåke subcellulære lokalisering og menneskehandel
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Utnytte pHluorin-merket reseptorer å overvåke subcellulære lokalisering og menneskehandel

Article DOI: 10.3791/55466-v • 09:59 min • March 16th, 2017
March 16th, 2017 •
1, 2, 1
1Department of Chemistry, University of Kentucky, 2Department of Biomedical Sciences, Marshall University

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Merking av ekstracellulære domenet til en membran protein med en pH-sensitive fluorofor, superecliptic pHluorin (SEP), kan subcellulære lokalisering, uttrykk og menneskehandel skal fastsettes. Imaging september-merkede proteiner med total indre refleksjon fluorescens mikroskopi (TIRFM) muliggjør kvantifisering av proteinnivåer i den perifere ER og plasmamembran.

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Biofysikk Superecliptic pHluorin reseptor membran trafficking subcellulære lokalisering plasma membran innsetting total intern refleksjon fluorescens mikroskopi membran protein oppregulering
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