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JoVE Journal Biochemistry
En Tailored HPLC rensing protokoll som gir høy renhet amyloid beta 42 og amyloid beta 40 peptider, i stand til oligomerdannelsen
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En Tailored HPLC rensing protokoll som gir høy renhet amyloid beta 42 og amyloid beta 40 peptider, i stand til oligomerdannelsen

Article DOI: 10.3791/55482-v • 06:34 min • March 27th, 2017
March 27th, 2017 •
1, 1, 1, 1
1Department of Chemistry, University of California, Santa Cruz

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Heri vi rapportere en skreddersydd HPLC rensing protokoll som gir høy renhet amyloid beta 42 (Aβ42) og amyloid beta 40 (Aβ40) peptider, i stand til oligomerdannelsen. Amyloid beta er en svært aggregering utsatt, hydrofobe peptid innblandet i Alzheimers sykdom. Den amyloidogene natur av peptidet som gjør dets rensing en utfordring.

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Biokjemi amyloid beta Peptide Rensing High Performance Liquid Chromatography Alzheimers sykdom oligomerdannelsen picup
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