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Developmental Biology

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In Vivo Imaging af transgene genekspression i Individuel Retinal progenitorer i kimære zebrafisk embryoner til Studieinformationen Cell Nonautonomous påvirkninger
 
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In Vivo Imaging af transgene genekspression i Individuel Retinal progenitorer i kimære zebrafisk embryoner til Studieinformationen Cell Nonautonomous påvirkninger

Article DOI: 10.3791/55490-v 10:36 min March 22nd, 2017
March 22nd, 2017

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Summary

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Live-sporing af individuelle WT retinale stamfædre i distinkte genetiske baggrunde giver mulighed for vurdering af bidraget fra celle non-autonome signalering under neurogenese. Her blev en kombination af gen knockdown, kimære generation via embryo transplantation og in vivo time-lapse konfokal billeddannelse anvendes til dette formål.

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Developmental Biology zebrafisk transplantation kimære levende billeddannelse celle non-autonome nethinde genekspression timing neurogenese
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