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Biochemistry

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JoVE Journal Biochemistry
Ein G-Quadruplex-DNA-Affinität Ansatz zur Reinigung von enzymatisch aktiven G4 Resolvase1
Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
Journal (Biochemistry)
Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
Affinity Chromatography Mediated Fibrinolytic Enzymes Purification from Crude Protein Extracts (Video) | JoVE
Journal (Biochemistry)
Affinity Chromatography Mediated Fibrinolytic Enzymes Purification from Crude Protein Extracts (Video) | JoVE
 
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Ein G-Quadruplex-DNA-Affinität Ansatz zur Reinigung von enzymatisch aktiven G4 Resolvase1

Article DOI: 10.3791/55496-v • 11:25 min • March 18th, 2017
March 18th, 2017 •
*1, *2, 3, 4, 1, 3
1Department of Cancer Biology, Wake Forest School of Medicine, 2YX Genomics, 3Department of Biology, Ball State University, 4Department of Hematology and Oncology, Roper St. Francis Hospital
* These authors contributed equally

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G4 Resolvase1 bindet an G-Quadruplex (G4) Strukturen mit dem engsten berichteten Affinität für ein G4-Bindungsprotein und stellt die Mehrheit der G4-DNA Abwickeln Aktivität in HeLa-Zellen. Wir beschreiben ein neuartiges Protokoll, das die Affinität und ATP-abhängige Abwickeln Aktivität von G4-Resolvase1 nutzt gezielt katalytisch aktive rekombinante G4R1 reinigen.

Tags

Biochemie Heft 121 G4 Resolvase1, G-Quadruplex ATP-abhängige Elution G-Quadruplex-Affinität Proteinreinigung DNA-Helikase RNA-Helikase
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