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Biochemistry

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JoVE Journal Biochemistry
En G-quadruplex DNA-affinitet tilnærming for rensing av enzymatisk aktivt G4 Resolvase1
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En G-quadruplex DNA-affinitet tilnærming for rensing av enzymatisk aktivt G4 Resolvase1

Article DOI: 10.3791/55496-v • 11:25 min • March 18th, 2017
March 18th, 2017 •
*1, *2, 3, 4, 1, 3
1Department of Cancer Biology, Wake Forest School of Medicine, 2YX Genomics, 3Department of Biology, Ball State University, 4Department of Hematology and Oncology, Roper St. Francis Hospital
* These authors contributed equally

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G4 Resolvase1 binder seg til G-quadruplex (G4) strukturer med de trangeste rapporterte affinitet for en G4-bindende protein og representerer flertallet av G4-DNA slappe aktivitet i HeLa celler. Vi beskriver en roman protokoll som utnytter affinitet og ATP-avhengig avvikling aktiviteten til G4-Resolvase1 å spesifikt rense katalytisk aktive rekombinant G4R1.

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Biokjemi G4 Resolvase1, G-quadruplex ATP-avhengige eluering G-quadruplex affinitet proteinrensing DNA helikase RNA helikase
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