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JoVE Journal Biochemistry
En G-quadruplex DNA-affinitet tilgang til oprensning af enzymatisk aktivt G4 Resolvase1
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En G-quadruplex DNA-affinitet tilgang til oprensning af enzymatisk aktivt G4 Resolvase1

Article DOI: 10.3791/55496-v • 11:25 min • March 18th, 2017
March 18th, 2017 •
*1, *2, 3, 4, 1, 3
1Department of Cancer Biology, Wake Forest School of Medicine, 2YX Genomics, 3Department of Biology, Ball State University, 4Department of Hematology and Oncology, Roper St. Francis Hospital
* These authors contributed equally

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G4 Resolvase1 binder til G-quadruplex (G4) strukturer med de strammeste rapporterede affinitet for et G4-bindende protein og repræsenterer størstedelen af ​​G4-DNA unwinding aktivitet i HeLa-celler. Vi beskriver en hidtil ukendt protokol, der udnytter affinitet og ATP afhængige afvikling aktivitet af G4-Resolvase1 til specifikt oprense katalytisk aktivt rekombinant G4R1.

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Biokemi G4 Resolvase1, G-quadruplex ATP-afhængig eluering G-quadruplex affinitet proteinoprensning DNA helicase RNA helicase
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