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Biochemistry

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JoVE Journal Biochemistry
Un approccio G-quadruplex del DNA-affinità per purificazione di enzimaticamente attiva G4 Resolvase1
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Un approccio G-quadruplex del DNA-affinità per purificazione di enzimaticamente attiva G4 Resolvase1

Article DOI: 10.3791/55496-v • 11:25 min • March 18th, 2017
March 18th, 2017 •
*1, *2, 3, 4, 1, 3
1Department of Cancer Biology, Wake Forest School of Medicine, 2YX Genomics, 3Department of Biology, Ball State University, 4Department of Hematology and Oncology, Roper St. Francis Hospital
* These authors contributed equally

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G4 Resolvase1 lega a strutture G-quadruplex (G4) con ristretti affinità segnalato per una proteina G4 vincolanti e rappresenta la maggioranza dell'attività svolgimento G4-DNA in cellule HeLa. Descriviamo un nuovo protocollo che sfrutta l'affinità e l'attività svolgimento ATP-dipendente di G4-Resolvase1 per purificare specificamente G4R1 ricombinante cataliticamente attivo.

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Biochimica G4 Resolvase1, G-quadruplex eluizione ATP-dipendente G-quadruplex purificazione di proteine ​​affinità DNA elicasi elicasi RNA
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