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Biochemistry

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JoVE Journal Biochemistry
En G-quadruplex DNA-affinitet metoden för rening av enzymatiskt aktiva G4 Resolvase1
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En G-quadruplex DNA-affinitet metoden för rening av enzymatiskt aktiva G4 Resolvase1

Article DOI: 10.3791/55496-v • 11:25 min • March 18th, 2017
March 18th, 2017 •
*1, *2, 3, 4, 1, 3
1Department of Cancer Biology, Wake Forest School of Medicine, 2YX Genomics, 3Department of Biology, Ball State University, 4Department of Hematology and Oncology, Roper St. Francis Hospital
* These authors contributed equally

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G4 Resolvase1 binder till G-quadruplex (G4) strukturer med de snävaste rapporterade affinitet för en G4-bindande protein och representerar majoriteten av G4-DNA avlindning aktivitet i HeLa-celler. Vi beskriver en ny protokoll som utnyttjar affinitet och ATP-beroende avveckling aktivitet av G4-Resolvase1 att specifikt rena katalytiskt aktivt rekombinant G4R1.

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Biochemistry G4 Resolvase1, G-quadruplex ATP-beroende eluering G-quadruplex affinitetsproteinrening DNA-helikas RNA-helikas
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