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Biochemistry

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JoVE Journal Biochemistry
Analyse af proteinkinaseaktivitet med radiomærket ATP
Capturing the Interaction Kinetics of an Ion Channel Protein with Small Molecules by the Bio-layer Interferometry Assay
Journal (Biochemistry)
Capturing the Interaction Kinetics of an Ion Channel Protein with Small Molecules by the Bio-layer Interferometry Assay
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Analyse af proteinkinaseaktivitet med radiomærket ATP

Article DOI: 10.3791/55504-v • 08:05 min • May 26th, 2017
May 26th, 2017 •
1, 1, 1
1Department of Pharmacology, University of Texas Southwestern Medical Center

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Proteinkinaser er højt udviklede signalerende enzymer og stilladser, der er kritiske for inter- og intracellulær signaltransduktion. Vi præsenterer en protokol til måling af kinaseaktivitet ved brug af radioaktivt mærket adenosintrifosfat ([γ-32P] ATP), en pålidelig metode til at hjælpe med at tydeliggøre cellesignalregulering.

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Biochemistry udgave 123 proteinkinase signaltransduktion biokemisk analyse radioaktivt mærket assay
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