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JoVE Journal
Biochemistry

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用放射性标记的ATP测定蛋白激酶活性
 
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用放射性标记的ATP测定蛋白激酶活性

Article DOI: 10.3791/55504-v 08:05 min May 26th, 2017
May 26th, 2017

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Summary

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Click here for the English version.

蛋白激酶是对于细胞间和细胞内信号转导至关重要的高度进化的信号酶和支架。我们提出了通过使用放射性标记的三磷酸腺苷([γ- 32 P] ATP)测量激酶活性的方案,这是一种有助于阐明细胞信号调节的可靠方法。

Tags

生物化学,第123期,蛋白激酶,信号转导,生物化学测定,放射性标记测定
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