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Journal / Neuroscience / zebrafisk
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Neuroscience

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Use of pHluorin to Assess the Dynamics of Axon Guidance Receptors in Cell Culture and in the Chick Embryo
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zebrafisk

Article DOI: 10.3791/55507-v • 08:24 min • April 18th, 2017
April 18th, 2017 •
1, 2, 1,2
1Department of Physiology and Biophysics, University of Colorado Anschutz Medical Campus (UCAMC), 2Neuroscience Graduate Program, University of Colorado Anschutz Medical Campus (UCAMC)

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Detta manuskript beskriver metoder för elektrofysiologiska registreringar från spinala neuroner i zebrafisk embryon och larver. Beredningen håller nervceller in situ och ofta innebär minimal dissektion. Dessa metoder medger för den elektrofysiologiska studier av en mängd olika spinala neuroner, från den inledande elektriska retbarhet förvärv genom de tidiga larvstadier.

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Neuroscience zebrafisk spinala neuroner motoriska neuroner sensoriska neuroner Rohon-Skägg cell CaP elektrofysiologi helcell patch clamp
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