Journal
/
Biology
/
Påvisning af ligand-aktiverede G-protein-koblede receptorer Internalisering af konfokal mikroskopi
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Detection of Ligand-activated G Protein-coupled Receptor Internalization by Confocal Microscopy

Påvisning af ligand-aktiverede G-protein-koblede receptorer Internalisering af konfokal mikroskopi

DOI:
10.3791/55514-v

•

10:24 min

•

April 09, 2017

•

, , , , ,
1National Engineering Research Center of Marine Facilities Aquaculture, Marine Science College,Zhejiang Ocean University, 2Institute of Biochemistry, College of Life Sciences,Zhejiang University

Chapters

  • 00:05Title
  • 00:49Cell Seeding
  • 02:05Cell Transfection Method 1 with Liposome Formulations
  • 03:44Cell Culture on Coverslips
  • 04:37Cellular Localization Using Confocal Microscopy
  • 05:47Cellular Internalization
  • 06:42Confocal Microscopy
  • 07:43Results: Gonadotropin Releasing Hormone Receptor Internalization Detection
  • 09:21Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokol beskriver konfokal mikroskopi påvisning af G-protein-koblet receptor (GPCR) internalisering i pattedyrceller. Det omfatter grundlæggende cellekultur, transfektion, og konfokal mikroskopi procedure og tilvejebringer en effektiv og let fortolkelige metode til at påvise den subcellulære lokalisering og internalisering af fusion-udtrykte GPCR.

Tags

GPCRLigand-activatedInternalizationConfocal MicroscopySubcellular LocalizationReceptor RecyclingHEK293 CellsTransfectionPlasmid DNAReduced Serum MediumReagentDMEMFBS

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Live Imaging of the Zebrafish Embryonic Brain by Confocal Microscopy

In vivo Quantification of G Protein Coupled Receptor Interactions using Spectrally Resolved Two-photon Microscopy

Imaging G-protein Coupled Receptor (GPCR)-mediated Signaling Events that Control Chemotaxis of Dictyostelium Discoideum

Kvantificering agonistaktivitet på G-protein-koblede receptorer

Genetisk kodede Molecular Probes at studere G-protein-koblede receptorer

Karakterisering af G-protein-koblede receptorer med en fluorescens-baserede calciummobilisering Assay

In vivo Klonale Sporing af hæmatopoietisk stamcelletransplantation og progenitorceller Præget af Fem fluorescerende proteiner ved hjælp af konfokal og multiphoton mikroskopi

In Vivo 4-Dimensional sporing af hæmatopoietisk stamcelletransplantation og stamcelle i Voksen Mouse calvarie knoglemarv

Visualisere Clathrin endocytose af G-protein-koblede receptorer på Single-begivenhed opløsning via TIRF Mikroskopi

Sporing lægemiddelinduceret Ændringer i Receptor Post-internalisering Trafficking af Colocalizational Analysis

Read Article