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Detectie van ligand-geactiveerde G-proteïne gekoppelde receptor internalisatie door confocale microscopie
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Detectie van ligand-geactiveerde G-proteïne gekoppelde receptor internalisatie door confocale microscopie

Article DOI: 10.3791/55514-v • 10:24 min • April 9th, 2017
April 9th, 2017 •
1, 1, 1, 1, 2, 1
1National Engineering Research Center of Marine Facilities Aquaculture, Marine Science College, Zhejiang Ocean University, 2Institute of Biochemistry, College of Life Sciences, Zhejiang University

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Dit protocol beschrijft confocale microscopie detectie van G-eiwit-gekoppelde receptor (GPCR) internalisatie in zoogdiercellen. Het omvat de basis-celkweek, transfectie, en confocale microscopie procedure en zorgt voor een efficiënte en gemakkelijk te interpreteren methode om de subcellulaire lokalisatie en de internalisering van-fusion uitgedrukt GPCR detecteren.

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Molecular Biology subcellulaire lokalisatie internalisatie GPCR GnRHR GFP confocale microscopie
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