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Påvisning af ligand-aktiverede G-protein-koblede receptorer Internalisering af konfokal mikroskopi
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Påvisning af ligand-aktiverede G-protein-koblede receptorer Internalisering af konfokal mikroskopi

Article DOI: 10.3791/55514-v • 10:24 min • April 9th, 2017
April 9th, 2017 •
1, 1, 1, 1, 2, 1
1National Engineering Research Center of Marine Facilities Aquaculture, Marine Science College, Zhejiang Ocean University, 2Institute of Biochemistry, College of Life Sciences, Zhejiang University

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Denne protokol beskriver konfokal mikroskopi påvisning af G-protein-koblet receptor (GPCR) internalisering i pattedyrceller. Det omfatter grundlæggende cellekultur, transfektion, og konfokal mikroskopi procedure og tilvejebringer en effektiv og let fortolkelige metode til at påvise den subcellulære lokalisering og internalisering af fusion-udtrykte GPCR.

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Molecular Biology subcellulær lokalisering internalisering GPCR GnRH-R GFP konfokal mikroskopi
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