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Journal / Developmental Biology / Protokoller til visualisering steroidogeniske…
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Developmental Biology

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JoVE Journal Developmental Biology
Protokoller til visualisering steroidogeniske organer og deres interaktive organer med Immunfarvning i bananfluen
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Protokoller til visualisering steroidogeniske organer og deres interaktive organer med Immunfarvning i bananfluen

Article DOI: 10.3791/55519-v • 12:10 min • April 14th, 2017
April 14th, 2017 •
*1, *1, *2, 3
1Graduate School of Life and Environmental Sciences, University of Tsukuba, 2Life Science Center of Tsukuba Advanced Research Alliance, University of Tsukuba, 3Faculty of Life and Environmental Sciences, University of Tsukuba
* These authors contributed equally

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Vi beskriver en protokol for dissektion, fiksering og immunfarvning af steroidogene organer i Drosophila larver og voksne kvinder at studere steroidhormon biosyntese og dens reguleringsmekanisme. Foruden steroidogene organer, vi visualisere innervation steroidogene organer samt steroidogene målceller såsom germlinie stamceller.

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Developmental Biology Developmental biologi neurovidenskab, Ecdysteroid biosyntese immunhistokemi kimlinie stamceller ovarie prothoracic kirtel
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