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Journal / Developmental Biology / Protocollen voor het visualiseren steroidogenic…
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JoVE Journal Developmental Biology
Protocollen voor het visualiseren steroidogenic organen en hun Interactive Organen met Immunokleuring in de fruitvlieg
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Protocollen voor het visualiseren steroidogenic organen en hun Interactive Organen met Immunokleuring in de fruitvlieg

Article DOI: 10.3791/55519-v • 12:10 min • April 14th, 2017
April 14th, 2017 •
*1, *1, *2, 3
1Graduate School of Life and Environmental Sciences, University of Tsukuba, 2Life Science Center of Tsukuba Advanced Research Alliance, University of Tsukuba, 3Faculty of Life and Environmental Sciences, University of Tsukuba
* These authors contributed equally

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We beschrijven een protocol voor dissectie, fixatie en immunokleuring van steroidogenic organen in Drosophila larven en volwassen vrouwtjes steroïde hormonen biosynthese en haar reguleringsmechanisme bestuderen. Naast steroïdogene organen visualiseren we de innervatie van steroidogenic organen en steroidogene doelcellen zoals kiemlijn stamcellen.

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Developmental Biology ontwikkelingsbiologie neurowetenschappen, Ecdysteroid biosynthese immunohistochemie kiembaan stamcellen eierstok schildklier prothoracale
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