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Journal / Developmental Biology / Sarkoma Hücreler Mezenkimal-Epitel Geçişler…
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Developmental Biology

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JoVE Journal Developmental Biology
Sarkoma Hücreler Mezenkimal-Epitel Geçişler endüklenmesi
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Sarkoma Hücreler Mezenkimal-Epitel Geçişler endüklenmesi

Article DOI: 10.3791/55520-v • 11:42 min • April 7th, 2017
April 7th, 2017 •
1, 1, 1, 1, 2, 3, 4, 1, 1, 5, 2, 4, 1
1Department of Medicine, Duke University, 2Department of Bioengineering, Rice University, 3Department of Molecular Genetics and Microbiology, Duke University, 4Solid Tumor Program and the Duke Prostate Center, Duke University Medical Center, 5Duke University Medical Center

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Biz burada mikroRNA-200 aile üyeleri ve grainyhead benzeri 2 (GRHL2) kombine ektopik ifadesine dayalı sarkom hücrelerinde mezenkimal-epitel geçişler (MET) uyarılması için bir hücre kültürü yöntemi sunuyoruz. Bu yöntem kanser agresiflik ve tedavileri hakkında fenotipik esneklik biyolojik etkilerini daha iyi anlamak için uygundur.

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Gelişimsel Biyoloji Sayı 122 Mezenkimal-epitel geçişler sarkom transfeksiyon mikroRNA-200 aile GRHL2 hücre kültürü epitel plastisite
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