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JoVE Journal
Developmental Biology

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Induktion af Mesenchymale-Epithelial Overgange i sarkomceller
 
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Induktion af Mesenchymale-Epithelial Overgange i sarkomceller

Article doi: 10.3791/55520
April 7th, 2017

Summary April 7th, 2017

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Vi præsenterer her en cellekultur fremgangsmåde til induktion mesenchymale-epitel overgange (MET) i sarkomceller fra kombinerede ektopisk ekspression af microRNA-200 familiemedlemmer og grainyhead-lignende 2 (GRHL2). Denne metode er velegnet til en bedre forståelse af den biologiske virkning af fænotypisk plasticitet på kræft aggressivitet og behandlinger.

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