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Developmental Biology

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JoVE Journal Developmental Biology
Induktion af Mesenchymale-Epithelial Overgange i sarkomceller
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Induktion af Mesenchymale-Epithelial Overgange i sarkomceller

Article DOI: 10.3791/55520-v • 11:42 min • April 7th, 2017
April 7th, 2017 •
1, 1, 1, 1, 2, 3, 4, 1, 1, 5, 2, 4, 1
1Department of Medicine, Duke University, 2Department of Bioengineering, Rice University, 3Department of Molecular Genetics and Microbiology, Duke University, 4Solid Tumor Program and the Duke Prostate Center, Duke University Medical Center, 5Duke University Medical Center

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Vi præsenterer her en cellekultur fremgangsmåde til induktion mesenchymale-epitel overgange (MET) i sarkomceller fra kombinerede ektopisk ekspression af microRNA-200 familiemedlemmer og grainyhead-lignende 2 (GRHL2). Denne metode er velegnet til en bedre forståelse af den biologiske virkning af fænotypisk plasticitet på kræft aggressivitet og behandlinger.

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Developmental Biology mesenchvmal-epitel overgange sarkom transfektion microRNA-200 familie GRHL2 cellekultur epitelial plasticitet
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