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JoVE Journal Medicine
Turbidimetry on Human Washed Platelets
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Turbidimetry on Human Washed Platelets: The Effect of the Pannexin1-inhibitor Brilliant Blue FCF on Collagen-induced Aggregation

Article DOI: 10.3791/55525-v • 09:13 min • April 6th, 2017
April 6th, 2017 •
1, 2, 2, 1
1Department of Pathology and Immunology, University of Geneva, 2Division of Angiology and Haemostasis & Geneva Platelet Group, Geneva University Hospitals

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Summary
Automatic Translation

We describe a straightforward method for the isolation of washed platelets from human blood followed by agonist-induced platelet aggregation measurements by turbidimetry. As an example we apply this method for studying the aggregation response of human platelets to collagen after a pre-incubation with the Pannexin1 channel inhibitor Brilliant Blue FCF.

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Turbidimetry Human Washed Platelets Pannexin1-inhibitor Brilliant Blue FCF Collagen-induced Aggregation Isolation Of Washed Platelets Agonist-induced Platelet Aggregation Measurements Platelet Biology Bleeding Disorders Light Transmission Aggregometry Citric Acid Monohydrate Trisodium Citrate Dihydrate Anhydrous D-glucose ACD Solution Tyrode's Buffer PH Adjustment Hydrogen Chloride Filter Sterilize
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