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JoVE Journal
Genetics

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适用于下一代测序的植物细菌DNA富集方法的优化与比较分析
 
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适用于下一代测序的植物细菌DNA富集方法的优化与比较分析

Article DOI: 10.3791/55528
July 28th, 2017

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Summary July 28th, 2017

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提出了两种植物细菌DNA富集方法的比较和优化:传统差速离心和基于甲基化状态的总gDNA分馏。我们评估产生的DNA数量和质量,证明在短读下一代测序中的性能,并讨论长读单分子测序中使用的潜力。

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