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JoVE Journal Genetics
Single Molecule Analyse van de Laser Gelokaliseerde Psoralen Adducten
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Single Molecule Analyse van de Laser Gelokaliseerde Psoralen Adducten

Article DOI: 10.3791/55541-v • 11:46 min • April 20th, 2017
April 20th, 2017 •
1, 1, 1, 1, 1, 1, 1
1Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health

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Lasers worden vaak gebruikt in studies van de cellulaire respons op DNA-schade. Echter, ze genereren lesies waarvan de afstand, frequentie en botsingen met replicatievorken worden zelden gekenmerkt. We beschrijven een benadering die de bepaling van deze parameters laser gelokaliseerde crosslinks tussen de strengen mogelijk maakt.

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Genetics DNA schade laser lokalisering DNA vezels InterStrand verknopen single molecule imaging
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