Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / 연골 세포 분열 분열의 DNA 메틸화 수준을 정량화하는 5-mC Dot Blot…
JoVE Journal
Developmental Biology

A subscription to JoVE is required to view this content.

JoVE Journal Developmental Biology
연골 세포 분열 분열의 DNA 메틸화 수준을 정량화하는 5-mC Dot Blot Assay
Ex Utero Electroporation and Organotypic Slice Culture of Mouse Hippocampal Tissue
Journal (Developmental Biology)
Ex Utero Electroporation and Organotypic Slice Culture of Mouse Hippocampal Tissue
Differentiating Chondrocytes from Peripheral Blood-derived Human Induced Pluripotent Stem Cells
Journal (Developmental Biology)
Differentiating Chondrocytes from Peripheral Blood-derived Human Induced Pluripotent Stem Cells
 
Click here for the English version

연골 세포 분열 분열의 DNA 메틸화 수준을 정량화하는 5-mC Dot Blot Assay

Article DOI: 10.3791/55565-v • 10:07 min • May 17th, 2017
May 17th, 2017 •
1,2, 3, 4,5, 2,6, 2, 2, 1,2
1Guangzhou Medical University, 2Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Laboratory of Digital Orthopeadic Engineering, Department of Orthopedics, Shenzhen Second People's Hospital (The First Hospital Affiliated to Shenzhen University), 3Department of Chemistry, The Chinese University of Hong Kong, 4School of Biomedical Engineering, Shanghai Jiao Tong University, 5Renji Hospital Clinical Stem Cell Research Center, Shanghai Jiao Tong University School of Medicine, 6Shantou University Medical College

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

우리는 5- 메틸 시토신 (5-mC) 도트 블롯을 기반으로 DNA 메틸화를 정량화하는 방법을 제시합니다. 우리는 연골 세포 탈분화 동안 5-mC 수준을 결정했다. 이 간단한 기술은 ACI 치료에서 연골 세포 표현형을 신속하게 결정하는데 사용될 수 있습니다.

Tags

발달 생물학 123 호 세포 배양 단층 확장 연골 세포 탈분화 DNA 메틸화 5-mC 도트 블롯 분석 게놈 DNA 추출
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter