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JoVE Journal Developmental Biology
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Ein 5-mC-Dot-Blot-Assay, der den DNA-Methylierungsgrad der Chondrozyten-Dedifferenzierung quantifiziert

Article DOI: 10.3791/55565-v • 10:07 min • May 17th, 2017
May 17th, 2017 •
1,2, 3, 4,5, 2,6, 2, 2, 1,2
1Guangzhou Medical University, 2Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Laboratory of Digital Orthopeadic Engineering, Department of Orthopedics, Shenzhen Second People's Hospital (The First Hospital Affiliated to Shenzhen University), 3Department of Chemistry, The Chinese University of Hong Kong, 4School of Biomedical Engineering, Shanghai Jiao Tong University, 5Renji Hospital Clinical Stem Cell Research Center, Shanghai Jiao Tong University School of Medicine, 6Shantou University Medical College

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Wir stellen ein Verfahren zur Quantifizierung der DNA-Methylierung auf der Basis des 5-Methylcytosin (5-mC) -Dot-Blots vor. Wir haben die 5-mC-Werte während der Chondrozyten-Dedifferenzierung bestimmt. Diese einfache Technik könnte verwendet werden, um schnell den Chondrozyten-Phänotyp in der ACI-Behandlung zu bestimmen.

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Entwicklungsbiologie Ausgabe 123 Zellkultur Monolayer-Expansion Chondrozyten-Dedifferenzierung DNA-Methylierung 5-mC-Dot-Blot-Analyse genomische DNA-Extraktion
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