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JoVE Journal Developmental Biology
Un test a blot di 5 mc quantificando il livello di metilazione del DNA della dedifferentiation di condrociti
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Un test a blot di 5 mc quantificando il livello di metilazione del DNA della dedifferentiation di condrociti

Article DOI: 10.3791/55565-v • 10:07 min • May 17th, 2017
May 17th, 2017 •
1,2, 3, 4,5, 2,6, 2, 2, 1,2
1Guangzhou Medical University, 2Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Laboratory of Digital Orthopeadic Engineering, Department of Orthopedics, Shenzhen Second People's Hospital (The First Hospital Affiliated to Shenzhen University), 3Department of Chemistry, The Chinese University of Hong Kong, 4School of Biomedical Engineering, Shanghai Jiao Tong University, 5Renji Hospital Clinical Stem Cell Research Center, Shanghai Jiao Tong University School of Medicine, 6Shantou University Medical College

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Presentiamo un metodo per quantificare la metilazione del DNA in base alla macchia dot-5-metilcitosina (5-mc). Abbiamo determinato i livelli di 5 mC durante la dedifferentiation dei condrociti. Questa semplice tecnica potrebbe essere utilizzata per determinare rapidamente il fenotipo condrocitico nel trattamento ACI.

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Biologia dello sviluppo Numero 123 Coltura cellulare espansione monostrato dedifferentiation condroscica metilazione del DNA analisi di macchia a punti da 5 mC estrazione genomica del DNA
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