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Journal / Developmental Biology / 在小鼠胚胎干细胞结构功能研究使用重组酶介导的盒式交换…
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Developmental Biology

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JoVE Journal Developmental Biology
在小鼠胚胎干细胞结构功能研究使用重组酶介导的盒式交换
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在小鼠胚胎干细胞结构功能研究使用重组酶介导的盒式交换

Article DOI: 10.3791/55575-v • 15:13 min • April 27th, 2017
April 27th, 2017 •
1,2,3,4, 1,2, 1,2,4, 1,2,4, 1,2, 5, 6, 1,2,4, 7, 1,2,4, 1,2,3,4
1Department of Biomedical Molecular Biology, Ghent University, 2Inflammation Research Center, VIB, 3Center for Medical Genetics, Ghent University Hospital, 4Cancer Research Institute Ghent (CRIG), 5Department of Basic Medical Sciences, Faculty of Medicine and Health Sciences, Ghent University, 6Helmholtz Center for Infection Research, 7Mammalian Functional Genetics Laboratory, Division of Blood Cancers, Australian Centre for Blood Diseases, Department of Clinical Haematology, Monash University and Alfred Health Alfred Centre

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蛋白质常含有可发挥不同的细胞功能多个域。基因敲除（KO）不考虑这个功能的多样性。这里，我们报告在KO胚胎干细胞的重组介导的盒式交换（RMCE）系的结构 - 功能的方法，其允许各种功能结构域或蛋白质的变体的分子清扫。

Tags

发育生物学，第122，结构 - 功能，小鼠胚胎干细胞，干细胞分离，pluripotin，重组介导的盒式交换，ROSA26定位，敲除，ROSA26基因座，胚状体，救援构建体
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