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Journal / Developmental Biology / Estudos de estrutura-função em células estaminais …
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JoVE Journal Developmental Biology
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Estudos de estrutura-função em células estaminais embrionárias Rato Usando recombinase mediada por troca cassete

Article DOI: 10.3791/55575 • 15:13 min
April 27th, 2017 •
1,2,3,4, 1,2, 1,2,4, 1,2,4, 1,2, 5, 6, 1,2,4, 7, 1,2,4, 1,2,3,4
1Department of Biomedical Molecular Biology, Ghent University, 2Inflammation Research Center, VIB, 3Center for Medical Genetics, Ghent University Hospital, 4Cancer Research Institute Ghent (CRIG), 5Department of Basic Medical Sciences, Faculty of Medicine and Health Sciences, Ghent University, 6Helmholtz Center for Infection Research, 7Mammalian Functional Genetics Laboratory, Division of Blood Cancers, Australian Centre for Blood Diseases, Department of Clinical Haematology, Monash University and Alfred Health Alfred Centre

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April 27th, 2017

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Proteínas, muitas vezes contêm vários domínios que podem exercer diferentes funções celulares. Gene knock-out (KO) não consideram essa diversidade funcional. Aqui, relatamos uma abordagem de estrutura-função com base na troca de cassete mediada por recombinação (RMCE) em KO células estaminais embrionárias que permite a dissecação molecular de vários domínios funcionais ou variantes de uma proteína.

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