Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / Struktur og funktion Studier i embryonale…
JoVE Journal
Developmental Biology

A subscription to JoVE is required to view this content.

JoVE Journal Developmental Biology
Struktur og funktion Studier i embryonale stamceller fra mus Brug rekombinase-medieret Cassette Exchange
Intravenous and Intra-amniotic In Utero Transplantation in the Murine Model
Journal (Developmental Biology)
Intravenous and Intra-amniotic In Utero Transplantation in the Murine Model
Aggregate Size Optimization in Microwells for Suspension-based Cardiac Differentiation of Human Pluripotent Stem Cells
Journal (Developmental Biology)
Aggregate Size Optimization in Microwells for Suspension-based Cardiac Differentiation of Human Pluripotent Stem Cells
 
Click here for the English version

Struktur og funktion Studier i embryonale stamceller fra mus Brug rekombinase-medieret Cassette Exchange

Article DOI: 10.3791/55575-v • 15:13 min • April 27th, 2017
April 27th, 2017 •
1,2,3,4, 1,2, 1,2,4, 1,2,4, 1,2, 5, 6, 1,2,4, 7, 1,2,4, 1,2,3,4
1Department of Biomedical Molecular Biology, Ghent University, 2Inflammation Research Center, VIB, 3Center for Medical Genetics, Ghent University Hospital, 4Cancer Research Institute Ghent (CRIG), 5Department of Basic Medical Sciences, Faculty of Medicine and Health Sciences, Ghent University, 6Helmholtz Center for Infection Research, 7Mammalian Functional Genetics Laboratory, Division of Blood Cancers, Australian Centre for Blood Diseases, Department of Clinical Haematology, Monash University and Alfred Health Alfred Centre

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Proteiner indeholder ofte flere domæner, som kan øve forskellige cellulære funktioner. Gene knock-outs (KO) anser ikke denne funktionel diversitet. Her rapporterer vi en rekombinationsmedieret kassette udveksling (RMCE) -baseret struktur-funktion tilgang i KO embryonale stamceller, som giver mulighed for den molekylære dissektion af forskellige funktionelle domæner eller varianter af et protein.

Tags

Developmental Biology struktur-funktion mus embryonale stamceller stamcellefaktor isolation pluripotin rekombinationsmedieret kassette udveksling ROSA26 målretning knock-out ROSA26 locus embryoide legemer redning konstruktioner
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter