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Developmental Biology

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JoVE Journal Developmental Biology
Struktur-funksjon studier i mus embryonale stamceller Bruke recombinase-mediert Cassette Veksling
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Struktur-funksjon studier i mus embryonale stamceller Bruke recombinase-mediert Cassette Veksling

Article DOI: 10.3791/55575-v • 15:13 min • April 27th, 2017
April 27th, 2017 •
1,2,3,4, 1,2, 1,2,4, 1,2,4, 1,2, 5, 6, 1,2,4, 7, 1,2,4, 1,2,3,4
1Department of Biomedical Molecular Biology, Ghent University, 2Inflammation Research Center, VIB, 3Center for Medical Genetics, Ghent University Hospital, 4Cancer Research Institute Ghent (CRIG), 5Department of Basic Medical Sciences, Faculty of Medicine and Health Sciences, Ghent University, 6Helmholtz Center for Infection Research, 7Mammalian Functional Genetics Laboratory, Division of Blood Cancers, Australian Centre for Blood Diseases, Department of Clinical Haematology, Monash University and Alfred Health Alfred Centre

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Proteiner inneholder ofte flere domener som kan utøve ulike cellulære funksjoner. Gene knock-outs (KO) betrakter ikke dette funksjonelle mangfold. Her rapporterer vi en rekombinasjon-mediert kassett utveksling (RMCE) -basert struktur-funksjonsmetoden i KO-embryonale stamceller som gjør det mulig for den molekylære disseksjon av forskjellige funksjonelle domener eller varianter av et protein.

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Developmental Biology utgave 122 struktur-funksjon muse-embryonale stamceller stamcelle isolasjon pluripotin rekombinasjon-mediert kassett utveksling ROSA26 målretting knock-out ROSA26 locus embryoidlegemer rednings konstruksjoner
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