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Análisis de suspensiones celulares aisladas de tejidos sólidos por Spectral Citometría de Flujo
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Análisis de suspensiones celulares aisladas de tejidos sólidos por Spectral Citometría de Flujo

Article DOI: 10.3791/55578-v • 11:08 min • May 5th, 2017
May 5th, 2017 •
1, 2,3,4,5, 2, 1
1Flow Cytometry Core Facility, Center for Translational Research-Technical Core, Institut Pasteur, 2Unit for Lymphopoiesis, Immunology Department, INSERM U1223, University Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, Institut Pasteur, 3Stem-Cell Microenvironments in Repair/Regeneration Team, Instituto de Investigação e Inovação em Saúde (i3s), INEB - Instituto de Engenharia Biomédica, 4ICBAS - Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, 5Stem Cells and Regenerative Medicine Team, UMRS 1166, ICAN - Institute of Cardiometabolism And Nutrition, UPMC - Université Pierre et Marie Curie - Paris 6, INSERM

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Este artículo describe espectral citometría, un nuevo enfoque en la citometría de flujo que utiliza las formas de los espectros de emisión para distinguir fluorocromos. Un algoritmo reemplaza compensaciones y puede tratar autofluorescencia como un parámetro independiente. Este nuevo enfoque permite el análisis adecuado de células aisladas de órganos sólidos.

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Biología Celular Número 123 citometría de flujo espectral auto-fluorescencia intestino corazón embrionario el análisis multi-paramétrico
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